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Validation of the RNA seq data by evaluation of a few genes by q-RT-PCR To validate the accuracy of our RNA-Seq data, we selected representative genes from different pathways for further analysis

Oral precursors generally work because they are easily absorbed into cells and converted to NAD+

Chronic metabolic conditions are characterized by: Increased production of reactive oxygen species Impaired antioxidant defenses Mitochondrial dysfunction Chronic inflammation Cellular damage to pancreatic -cells, adipocytes, and hepatocytes How GLP-1 Affects Oxidative Stress Research has revealed that GLP-1 receptor agonists exert antioxidant effects through several mechanisms: Direct antioxidant signaling: Activation of the GLP-1 receptor triggers cellular pathways that reduce ROS production Anti-inflammatory effects: Decreases production of pro-inflammatory cytokines that contribute to oxidative stress Mitochondrial protection: Improves mitochondrial function and reduces oxidative damage Endothelial function: Enhances nitric oxide production and vascular health However, during weight loss especially rapid weight loss induced by GLP-1 RAs patients may experience increased oxidative stress due to the mobilization of stored toxins from adipose tissue

There is ample evidence that bilirubin is a molecule with multiple biological functions, particularly antioxidant, signalling/hormonal and immunomodulatory effects